enzyme-linked相关论文
Aflatoxin Contamination of Garri Sold in Some Selected Markets in Benue State, North Central, Nigeri
The presence of aflatoxin-producing fungi in foods consumed by humans and animals has often resulted in the health hazar......
Cyanidin-horseradish peroxidase-hydroperoxide reaction system and its application in enzyme-linked i
A cyanidin-based horseradish peroxidase(HRP)-catalyzed reaction system was established in this work.In B-R buffer soluti......
目的 分析超声引导下胸膜活检联合外周血结核感染T细胞斑点试验(T-SPOT.TB)对结核性胸膜炎的诊断价值.方法 采用随机数字表法随机......
目的 建立检测乙型脑炎病毒抗体的抗体捕获ELISA方法.方法 以乙型脑炎病毒E蛋白抗原表位E39特异的单克隆抗体包被ELISA板,吸附乙型......
目的 了解新生儿单纯疱疹病毒1型(herpes simplex virus type 1,HSV-1)和人巨细胞病毒( human cytomegalovirus,HCMV)血清抗体阳性......
目的探讨酶联免疫吸附试验用于艾滋病筛检人群人类免疫缺陷病毒(HIV)抗体中的临床价值,为完善疾控中心HIV检测咨询工作提供参考依......
由于测量误差的存在,测量值与真值总存在一定程度的偏离,把对测量值不能肯定的程度定义为不确定度。不确定度是测量结果质量的重要指......
目的:了解屠宰生猪中甲型肝炎的流行情况,探讨猪作为HAV另一宿主和携带者的可能性,以期为人类甲肝的防治提供一定的理论依据。方法:采......
为了解孕期强化免疫对母血和脐血特异性抗体水平的影响,将门诊48例孕28~34周的孕妇平均分为研究组和对照组。研究组含服脊髓灰质炎活疫苗。......
本研究应用酶联免疫印清技术分析了东毕血吸虫成虫部分纯化抗原中具有免疫活性的蛋白质组分,其中分子量为35、80、92和94kD的蛋白质具有较灵敏......
Preliminary study of the diagnosis of pancreatic cancer with a serum pancreatic cancer-associated an
Preliminarystudyofthediagnosisofpancreaticcancerwithaserumpancreaticcancer-associatedantigenZhaoXiaoyan(赵晓晏);YuShiyuan(于世远);G.........
本文对斑点免疫结合试验的影响因素进行了探讨。在完全国产试剂条件下,阻断液的选择特别重要。并确定了阻断液、洗涤液、稀释液的......
目的探讨白细胞介素17(interleukin-17,IL-17)和IL-27在变应性鼻炎(AR)发病机制中的作用及其临床意义。方法采用酶联免疫吸附试验检测A......
INTRODUCTIONVitamin E succinate (RRR-α-Tocopheryl Succinate,VES), a derivative of natural vitamin E, is acompound ester......
AIM:To analyze hepatitis C virus(HCV)-specific immune responses in chronically infected patients under triple therapy wi......
Glypican-3 (GPC3) is a cell surface oncofetal proteoglycanthat is anchored by glycosylphosphatidylinositol.Whereas GPC3 is......
目的比较MEIA法与ELISA法检NHCV抗体的结果,以及2种方法在实际应用中的区别。方法收集152例ELISA法检NHCV抗体阳性的血清标本,并采用......
本试验以80只300日龄的A品系蛋鸡为试验对象,分5个日龄段按翅号采集蛋清、泄殖腔棉拭子,无菌抗凝血和血清。用ALV p27抗原检测试剂......
目的建立一种ELISA方法用于定量分析血清抗甲状腺过氧化物酶(TPO)抗体(TPOAb)。方法用生物素化牛血清清蛋白(BSA)和链霉亲合素包被微孔板......
目的利用基因工程表达的组氨酰转移核糖核酸合成酶自身抗原Jo-1蛋白,建立ELISA法,初步用于检测多发性肌炎/皮肌炎(PM/DM)中的抗Jo—1抗体......
Anti-bungarotoxin anti-serum,which has the internal image of nicotinicacetylcholine receptor,was used as a tool to measu......
目的探讨冠心病患者血清对氧磷酯酶1(PON1)活性变化与氧化型低密度脂蛋白(oxLDL)水平的关系.方法用分光光度法测定99例冠心病患者[......
目的 定性检测胃癌患者外周血CEA(癌胚抗原)mRNA表达,对预测胃癌微转移的意义。方法 应用RT-PCR(U降转录-聚合酶链反应)ELISA(酶联免疫......
The present study examined von Willebrand factor (vWF) levels and ADAMTS13 activity in pregnant and severe preeclamptic ......
建立梅毒螺旋体重组抗原酶联免疫吸附试验 (ELISA),用于梅毒血清学诊断和调查。其法,用表达的重组抗原TPN17和TmpA,建立检测血清特异......
Quantitative determination of erlotinib in human serum using competitive enzyme-linked immunosorbent
A selective and sensitive competitive enzyme-linked immunosorbent assay(ELISA)method was developed and validated for the......
用微量ELISA法检测807例份不同年龄、性别的健康人血清中水痘-带状疱疹病毒疱疹病毒IgG抗体,0-1岁年龄组最低,为48.15%,25-35岁年龄达到高......
【正】Objective:To develop a standard enzyme-linked immunosorbent assay(ELJSA) for the detection of bovine herpesvirus typ......
Development of a competitive enzyme-linked immunosorbent assay for therapeutic drug monitoring of af
Afatinib is an oral tyrosine kinase inhibitor (TKI) approved for treating advanced non-small cell lung cancer. It is nec......
用戊二醛将L-同型磺基丙氨酸(L-HCA)连接到牛血清白蛋白(BSA),形成L-HCA-BSA连接物.用L-HCA-BSA免疫小鼠后,收集脾淋巴细胞,并与骨......
目的探讨间接免疫荧光法(IIFA)和酶联免疫吸附试验(ELISA)检测抗核抗体(ANA)和抗双链DNA(ds-DNA)抗体的异同。方法抽取确诊或疑为自身免疫......
Two rapid,sensitive and reliable immunoassay methods,namely competitive indirect enzyme-linked immunosorbent assay(CI- E......
目的探讨乙型肝炎病毒(HBV)感染与白细胞介素27(IL-27)的相关性,并在体外通过RT-PCR基因表达探讨HBV对IL-27水平的影响。方法采用酶联免......
Enzyme-linked immunosorbent assay (ELISA) is the most widely used method for measuring a single cytokine. Recent develop......
Detectionofanti-HEVbysyntheticpeptidesPanWei(潘卫),YuChao(俞超),QiZhongtian(戚中田),CuiDafu(崔大敷),SongYanbing(宋燕斌),CuiHenran(崔恒苒),Zho.........
目的建立双单抗夹心酶联免疫吸附试验法用于测定早孕因子(EPF).方法筛选可稳定分泌抗EPF单克隆抗体的细胞株,制备腹水型单抗(McAb)......
Determnation of ochratoxin A in grain by monoclonal antibody-based enzyme-linked immunosorbent assay
DetermnationofochratoxinAingrainbymonoclonalantibody-basedenzyme-linkedimmunosorbentassayYangChuanhe;LuoXueyun;LiuChang;LiWen.........
提出一种新的HRP底物-硝基磺酚S,初步探讨了在HRP催化下被过氧化氢氧化的机制,以硝基磺酚S为底物测定了酶促反应动力学常数Km和Ks分别为4.51×10^-5mol/L和28.1mol/L&#......
采用细胞融合技术制备抗赤潮毒素腹泻性贝毒软海绵酸的单克隆抗体,应用此抗体发展建立了软海绵酸的间接竞争酶联免疫检测方法。采......
AIM To assess serum cartilage oligomeric matrixprotein (COMP) as a marker of cirrhosis and risk ofprogression to hepatocel......
Objective: Leptospirosis is a known zoonotic disease which is closely related to certain high-risk occupational group. T......
The purpose of this study is to compare the levels of IL-6, 8 in tear fluids of people exposed to mustard gas in the war......
载脂蛋白(a)〔apo(a)〕免疫BALB/c小鼠,应用杂交瘤技术制备了17株抗apo(a)单克隆抗体(McAb),其中4株与人血清Lp(a)反应良好,且不与低密度脂蛋白(LDL)和纤溶酶原(Pmg)反应。将这4株McAb等量......